Targeting of cholera toxin A (ctxA) gene by zinc finger nuclease: pitfalls of using gene editing tools in prokaryotes
نویسندگان
چکیده
منابع مشابه
Dissection of Splicing Regulation at an Endogenous Locus by Zinc-Finger Nuclease-Mediated Gene Editing
Sequences governing RNA splicing are difficult to study in situ due to the great difficulty of traditional targeted mutagenesis. Zinc-finger nuclease (ZFN) technology allows for the rapid and efficient introduction of site-specific mutations into mammalian chromosomes. Using a ZFN pair along with a donor plasmid to manipulate the outcomes of DNA repair, we introduced several discrete, targeted ...
متن کاملGenetic analysis of zinc-finger nuclease-induced gene targeting in Drosophila.
Using zinc-finger nucleases (ZFNs) to cleave the chromosomal target, we have achieved high frequencies of gene targeting in the Drosophila germline. Both local mutagenesis through nonhomologous end joining (NHEJ) and gene replacement via homologous recombination (HR) are stimulated by target cleavage. In this study we investigated the mechanisms that underlie these processes, using materials fo...
متن کاملGene editing using a zinc-finger nuclease mimicking the CCR5Δ32 mutation induces resistance to CCR5-using HIV-1.
OBJECTIVES To characterize a new zinc-finger nuclease (ZFN) that targets close to the sequence of the 32 bp deletion polymorphism in the CCR5 gene, and to generate cells resistant to HIV-1 strains that use CCR5. CCR5Δ32 is a naturally occurring deletion that provides genetic resistance to R5-tropic HIV-1. The specificity and efficacy of a newly identified target for CCR5 gene editing, near the ...
متن کاملGenetic analysis of zinc-finger nuclease-induced gene
Zinc-finger nucleases can be designed to cleave arbitrary genomic sequences, which stimulates localized mutagenesis and targeted gene replacement. Both outcomes depend on normal cellular processes of DNA repair, and this study examines the pathways responsible. Gene replacement by homologous recombination depends on the activity of the Rad51 protein. Much of local mutagenesis depends on DNA lig...
متن کاملGene Targeting in NOD Mouse Embryos Using Zinc-Finger Nucleases
Studies in NOD mice have provided important insight into the genetics and pathogenesis of type 1 diabetes (T1D). Our goal was to further explore novel methods of genetic manipulation in this mouse model. We tested the feasibility of using zinc-finger nucleases (ZFNs) to knock out a gene directly in a pure NOD background, bypassing the need of embryonic stem cells. We report here the successful ...
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ژورنال
عنوان ژورنال: Research in Pharmaceutical Sciences
سال: 2020
ISSN: 1735-5362
DOI: 10.4103/1735-5362.283818